Is Reversing Age Really Possible?

The most recent breakthrough in age reversal in rats

Image for post
Image for post
Image for post
Image for post
I wish I could say the same…
Image for post
Image for post
That’s a hell of a punchline

What The Hell Am I Talking About?!?!

Let me explain.

Image for post
Image for post

Setting the Scene

So, the idea behind this experiment isn’t completely novel. In fact, there have been previous experiments testing similar ideas.

Image for post
Image for post
I know. That’s gross. But, believe me, it’s even worse if you see pictures

Designing Epigenetic Clocks

Now, before we even start testing the treatment, we have to determine a way to calculate their age biologically (since their chronological age will remain the same).

  • The brain clock was trained on samples from the whole brain, hippocampus, hypothalamus, neocortex, substantia nigra, cerebellum, and the pituitary gland.
  • The liver and blood clocks were trained on the liver and blood, respectively
  • 2 human-rat clocks applied to both humans and rats
    - One measured absolute age (in terms of years)
    - The other measured relative age, as a ratio of chronological age to the max lifespan, with values between 0 and 1. This ratio allowed alignment and comparison between species with very different lifespans (like humans and rats), which is just not possible with the absolute age clock.
Image for post
Image for post
As seen in all the graphs, the chronological age and the DNAm age of the organisms had a high correlation, proving that the epigenetic clocks carried out their function effectively

Hypothesis and Experimental Plan

The researchers hypothesized that plasma fraction treatment would reduce epigenetic age in the 2 year old rats they were using for the experiment.

  • group of 6 old, untreated rats (109 weeks, or around 2 years, old)
  • group of 6 plasma-fraction treated old rats (also 109 weeks old)
Image for post
Image for post
Spraque Dawley Rats… essentially the stereotypical lab rats

Hematological and Biochemical Analysis

During the course of the experiment, blood was regularly drawn from the mice for hematological and biochemical analysis to monitor the impact of the treatment on the blood and vital organs.

Testing Cognitive Function

The researchers used a Barnes maze to measure the period required by rats to escape through the right hole, also known as the latency period. This depends on the ability of the rats to remember which holes they have already checked, and the result they got from that.

Image for post
Image for post
A Barnes maze — there’s a bunch of holes, and only one of them contains the “escape box”

Measuring Cellular Stress

To determine the effect the treatment had on cellular stress, the researchers considered two of the main types/results of cellular stress: oxidative stress and chronic inflammation.

And What Did They Find?

Let’s talk about the results from the epigenetic clocks first.

Image for post
Image for post

What Does This Mean?

Well, first off, this definitely does not mean you should run into the nearest hospital and ask them to inject you with the blood of some youngster. In fact, if you live in the United States, you don’t even have this option, since the FDA banned all plasma fraction treatment experiments.

Image for post
Image for post
How exactly would that conversation with your doctor go 🤔?

18 y/o innovator working on reversing ageing and researching cancer vaccines. www.akshajdarbar.com